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The concentrations of the various macrospecies comprise the sum of the concentration of those microspecies that contain the same number of protons, for example in GSH: Determination of the redox potential of the GSSG/GSH system was achieved indirectly, applying a redox couple with a known standard redox potential

Izquierdo-Altarejos P, Arenas YM, Montoliu C, Prez-Martnez G, Llansola M, Felipo V

Reaction Development and Optimization Small-scale reaction scouting is used to refine stoichiometry, solvent system, pH window, and reaction time so the chosen route gives a practical balance of selectivity, conversion, and stability

There is no enhancement on the subtraction image (Post-Gd minus Pre-Gd)